A. a
B. Aa
C. aa
D. AA
The unknown genotype is Aa. This can be inferred from the Punnet square, which shows that half of the offspring are Aa and half are aa. This indicates that the unknown parent must have one dominant allele (A) and one recessive allele (a), making its genotype Aa.
The other options are not correct because they do not match the results shown in the Punnet square. If the unknown genotype was aa or AA, all of the offspring would have the same genotype as their parent. If the unknown genotype was a, it would not be a valid genotype as it only has one allele.
Therefore, the Correct Answer is B.